Accurate determination of a protein’s charge heterogeneity helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of a protein’s isoelectric point (pI). In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pI is formed.

The PA 800 Plus Pharmaceutical Analysis System automates the advanced cIEF technology necessary to successfully achieve high precision and quantitative separations. The use of optimized universal methods and synthetic pI markers attains the highest levels of precision in pI calculation and direct isoform quantitation with on-line UV detection.

This kit provides reagent volumes for analysis of 100 samples and includes:
• eCap Neutral Capillary, 1 unit
• cIEF gel
• cIEF Peptide Marker Kit, 5 vials (pI 4.1, pI 5.5, pI 7.0, pI 9.5, pI 10.0), 240 µL each
• Sample Loading Solution (SLS), 6 mL
• CE Grade Water

NOTE: Additional reagents from other vendors are required for this application. Methods and necessary supplies can be referenced in the following AIBs, available for download at www.sciex.com:
• A-11634: Identification of System Parameters Critical for High-Performance cIEF
• A-12015: A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range
• A-12026: High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

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