Reagents for the detection of complement components and IgG antibodies on human red cells or the detection of red cell antibodies in human serum
Pelikloon polyspecific anti-human serum contains polyclonal IgG and monoclonal anti-C3d antibodies. For reasons of a visible control on the addition of the polyspecific anti-human serum during the test this product is also supplied as a green coloured reagent. Anti-IgG is prepared by immunising goats with purified Fc fragments of human IgG. Mouse anti-C3d monoclonal antibodies are produced as culture supernatants from stable hybridoma cell lines as first described by Köhler and Milstein (Nature 1975). These reagents meet the requirements of the concerned standards and guidelines. Performance characteristics are mentioned in the release documents, which are supplied with the products upon request. The principle of the test is the agglutination technique. These reagents can be used in direct antiglobulin test (DAT), for antibody detection and identification and for compatibility testing (crossmatch) in the indirect antiglobulin test (IAT). The inclusion of a positive control with each series of tests in the IAT is strongly recommended.