Blood grouping reagents for the detection of the Rhesus antigens C, c, E or e on human red cells
Pelikloon anti-C, c, E, and both e (IgM) monoclonal blood grouping reagents (clone number is mentioned on the corresponding certificate of analysis/release document and product label) are prepared from culture supernatant from stable hybridoma cell lines as first described by Köhler and Milstein (Nature 1975). Two different lines of Pelikloon reagents (varying in clone composition) are available and, if desired, can be used for confirmation of test results obtained with either line of reagents. These monoclonal reagents contain human IgM antibodies and have been specially selected and developed to provide a reliable alternative to polyclonal reagents. These reagents meet the requirements of the concerned standards and guidelines. Performance characteristics are mentioned in the release documents, which are supplied with the products upon request. The principle of the test is the agglutination technique, which is based on antigen/antibody reaction. These reagents can be used in either spin tube or microplate method. The inclusion of positive and negative controls with each series of blood group determinations is strongly recommended.