Capillary Electrophoresis

Onze oplossingen 

Ervaar de kracht en snelheid van SCIEX scheidingstechnologie
SCIEX capillaire elektroforese-instrumenten zijn ontwikkeld om uw analytische uitdagingen van vandaag aan te gaan, met één oog gericht op de eisen van morgen.

Of uw lab nu kloonselectie, geautomatiseerde en kwantitatieve analyse van biologische producten, proteomisch, metabolomisch of genomisch onderzoek of voedsel- en milieutests uitvoert, SCIEX heeft zeker een state-of-the-art oplossing voor u.

Lees meer over onze capillaire elektroforese-instrumenten en zie waarom wetenschappers over de hele wereld vertrouwen op SCIEX-technologie om hun onderzoek naar de toekomst te brengen.

Neem contact met ons op om te bespreken welk instrument het beste past bij jouw wensen en budget.

sciex BioPhase 8800 systeem

BioPhase 8800 system

Innovatief systeem dat parallelle verwerking van 8 monsters tegelijk mogelijk maakt en tegelijkertijd gevoelige CE-SDS en CIEF analyse levert. Zorgt voor compromisloze nauwkeurigheid en snellere antwoordtijden in het hele biologische ontwikkelingscontinuüm.

Meer informatie

sciex PA 800 Plus farmaceutisch analysesysteem

PA 800 Plus Pharmaceutical Analysis System

Flexibel platform voor meerdere analytische assays met een capillair met temperatuurregeling voor de grootst mogelijke reproduceerbaarheid en betrouwbaarheid van gegevens.

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sciex CESI 8000 Plus ESI-MS-systeem met hoge prestaties

CESI 8000 Plus ESI-MS-High-Performance System

Integreert capillaire elektroforese en elektrospray ionisatie (ESI) in één dynamisch proces. Verhoogt de scheidingsefficiëntie, snelheid en gevoeligheid aanzienlijk.

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sciex P/ACE™ MDQ Plus

P/ACE™ MDQ Plus

Geautomatiseerde, programmeerbare capillaire elektroforesesystemen ontworpen om snelle scheidingen uit te voeren van complexe monsters.

Meer informatie

sciex GenomeLab GeXP™ genetisch analysesysteem

GenomeLab GeXP™ Genetic Analysis System

Al je genetische analyses in één instrument. De GenomeLab GeXP ondersteunt verschillende toepassingen, waaronder DNA-sequentiebepaling, fragmentanalyse en multiplex genexpressie.

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Our products

SCIEX

Advanced cIEF Starter Kit

Reference : ABS-A80976

Accurate determination of a protein’s charge heterogeneity helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of a protein’s isoelectric point (pI). In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pI is formed. 

The PA 800 Plus Pharmaceutical Analysis System automates the advanced cIEF technology necessary to successfully achieve high precision and quantitative separations. The use of optimized universal methods and synthetic pI markers attains the highest levels of precision in pI calculation and direct isoform quantitation with on-line UV detection.

This kit provides reagent volumes for analysis of 100 samples and includes: 
• eCap Neutral Capillary, 1 unit 
• cIEF gel 
• cIEF Peptide Marker Kit, 5 vials (pI 4.1, pI 5.5, pI 7.0, pI 9.5, pI 10.0), 240 µL each 
• Sample Loading Solution (SLS), 6 mL 
• CE Grade Water 
 
 
NOTE: Additional reagents from other vendors are required for this application. Methods and necessary supplies can be referenced in the following AIBs, available for download at www.sciex.com:
• A-11634: Identification of System Parameters Critical for High-Performance cIEF
• A-12015: A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range
• A-12026: High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

SCIEX
P/ACE MDQ Plus Capillary Electrophoresis System
Reference : ABS-B52521

The P/ACE MDQ Plus is an automated, programmable capillary electrophoresis system designed to perform fast separations from complex samples.

The system includes:
> a selectable-wavelength high sensitivity modular UV/Vis detector (includes 200, 214, 230 and 254 nm filters to optimize analyte specificity) with UV source optics,
> temperature-controlled sample storage module
> 32 Karat™ Software configured on a desktop controller
> Installation Qualification, Operation Qualification 1 (OQ1) and documentation to aid in software validation are also included.

If required:
Add application support and training when desired.
Add other detectors specific for your application when desired - Optional DAD and/or LIF

Operational Qualification (OQ3) is NOT available for this system.

SCIEX
PA800 Plus Pharmaceutical Analysis CE System
Reference : ABS-A66528

The PA 800 Plus Pharmaceutical Analysis system provides a comprehensive, automated, and quantitative solution for the characterization and analysis of proteins. Innovative system design ensures dependable operation and durability. The PA 800 Plus application menu includes SDS-gel molecular weight analysis, IgG Purity and Heterogeneity analysis, charge heterogeneity analysis utilizing advanced capillary isoelectric focusing, and carbohydrate profiling for the assessment of glycoprotein microheterogeneity.
Modular detector design provides for the simple interchange of detectors.

The PA 800 Plus System includes:
• High-resolution separation module
• High-sensitivity UV, PDA, and solid-state laser-induced fluorescence detection
• Sample storage temperature control
• High-speed system controller with 32 Karat™ Version 10.3 on Win10
• Validated applications for SDS-MW, IgG Purity and Heterogeneity, cIEF, and glycan analysis
• Starter kit containing necessary hardware supplies to operate the system
• Installation Qualification (IQ) and Operational Qualification (OQ1) of the system are included.

If required
P/N A81010 must be added to the order for system start-up and installation.
System application installation and training must be added when desired.
Operational Qualification (OQ3) must be added for complete system validation.

SCIEX
PA800S Plus Pharmaceutical Analysis CE System
Reference : ABS-A66527

A subset of the PA 800 Plus Pharmaceutical Analysis system, the PA 800s Plus provides an automated and quantitative solution for the analysis of protein purity and molecular weight utilizing SDS-gel capillary electrophoresis and IgG Purity and Heterogeneity applications. The innovative system design ensures dependable operation and durability.

The PA 800s system includes:
• High-resolution separation module
• High-sensitivity PDA detection
• Sample storage temperature control
• High-speed system controller with 32 Karat™ Version 10.3 on Win10
• Validated applications for SDS-MW and IgG Purity and Heterogeneity
• Starter kit containing necessary hardware supplies to operate the system is included
• Installation Qualification (IQ) and Operational Qualification (OQ1) of the system are included.

If required:
P/N A81111 must be added to the order for system start-up and installation.
System application installation and training must be added when desired.
Operational Qualification (OQ3) must be added for complete system validation.

SCIEX
10kDa Internal Standard
Reference : ABS-A26487

For use with the IgG Purity/Heterogeneity and SDS MW Kits.

10 kDa internal standard is used as a mobility marker and all protein mobility is calculated relative to this internal standard.

SCIEX
Advanced cIEF Starter Kit
Reference : ABS-A80976

Accurate determination of a protein’s charge heterogeneity helps establish identity and stability. Capillary Isoelectric Focusing (cIEF) is a powerful technique that allows quantitative analysis of a protein’s isoelectric point (pI). In cIEF, a mixture of sample and ampholyte is introduced into a capillary and subjected to electrophoretic separation. In this process, a pH gradient through which analytes migrate to their respective pI is formed. 

The PA 800 Plus Pharmaceutical Analysis System automates the advanced cIEF technology necessary to successfully achieve high precision and quantitative separations. The use of optimized universal methods and synthetic pI markers attains the highest levels of precision in pI calculation and direct isoform quantitation with on-line UV detection.

This kit provides reagent volumes for analysis of 100 samples and includes: 
• eCap Neutral Capillary, 1 unit 
• cIEF gel 
• cIEF Peptide Marker Kit, 5 vials (pI 4.1, pI 5.5, pI 7.0, pI 9.5, pI 10.0), 240 µL each 
• Sample Loading Solution (SLS), 6 mL 
• CE Grade Water 
 
 
NOTE: Additional reagents from other vendors are required for this application. Methods and necessary supplies can be referenced in the following AIBs, available for download at www.sciex.com:
• A-11634: Identification of System Parameters Critical for High-Performance cIEF
• A-12015: A Robust cIEF Method: Intermediate Precision for the pH 5-7 Range
• A-12026: High-Resolution cIEF of Therapeutic Monoclonal Antibodies: A Platform Method Covering pH 4-10

SCIEX
Coolant for capillary cartridge liquid cooling, 450ML.
Reference : ABS-359976

Cooling liquid refill for use on temperature controlled instruments, for all Sciex CE systems including the BioPhase, PA 800 Plus, CESI 8000 Plus and P/ACE MDQ instruments (Qty: 1).

SCIEX
IgG Control Standard - 3 Pack
Reference : ABS-391734

Kit Component of P/N 390953 and P/N A10663 (IgG Purity/Heterogeneity Kit.)
For use as an experimental control when separating reduced or non-reduced IgG, with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns.

SCIEX
IgG Purity/Heterogeniety Assay Kit
Reference : ABS-A10663

Includes IgG Control Standard 1-pack and all chemistries.
The IgG Purity/Heterogeneity Assay has been developed for researchers employed in industrial biotechnology who are developing and manufacturing IgG reagents for research, diagnostic and therapeutic use. 

This assay has been specified for use on the PA 800 Plus to assess the purity and heterogeneity of IgG reagents in both a reduced and non-reduced state. The methodology involves heat-denaturing a specified concentration of IgG (both reduced and non-reduced) in the presence of SDS, and separating these proteins by size using high-resolution capillary gel electrophoresis technology. This assay will detect impurities as low as 0.1% and includes an IgG control with a designated quantity of non-glycosylated heavy chain to test both the resolution and quantitation suitability of the assay prior to running unknowns
 
Assay chemistry includes:
• Separation Capillary, 57 cm x 50 µm ID bare fused-silica, (2)
• SDS Gel Separation Buffer (proprietary formulation), 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• IgG Control Standard, 1 mg/mL in SDS sample buffer , 1 mL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• IgG Purity and Heterogeneity Analysis Guide, (1)

SCIEX
LIF Performance Test Mix
Reference : ABS-726022

LIF Test Mix, Sodium Fluorescein (20 mL). Test mix for testing the Laser-Induced Fluorescence (LIF) detector and determining the calibration correction factor (CCF) for the system.

SCIEX
Molecular Weight Sizing Standard - 3 Pack
Reference : ABS-A22196

Molecular Weight standards (10 to 225 KDa), 16mg/mL (100 µL) for use with the SDS-MW Kit (Part Number 390953) to provide a sizing ladder to reference proteins of interest against.

SCIEX
Pre-Assembled Capillary Cartridge
Reference : ABS-A55625

No more need to mount capillaries. Pre-assembled EZ-CE cartridge (part # A55625) for multiple applications (Purity Analysis, Rapid Charge Variant Analysis, and Fast Glycan Analysis)
> Precision cut tips – ensure uniformity of injection plug from capillary to capillary
> Alignment precision at window – reduces potential failure due to misalignment
> Reduce preparation time for operator – simplifying the process and training requirements

SCIEX
Pre-cut N-CHO Coated Capillary 50 µm x 65 cm
Reference : ABS-477601
N-CHO (PVA) Capillary with laser burned window - 50 µm ID x 65 cm total length.
Capillaries must be trimmed to optimize speed/resolution.
This capillary is used with the Carbohydrate Labeling and Analysis Kit.
Reorder component for P/N 477600.