Capillary Electrophoresis

eCAP™ Neutral Coated Capillary with laser burned window - 50 µm ID x 67 cm total length.
Capillary used with the Advanced cIEF Starter Kit (Part number A80976).
Capillaries must be trimmed to optimize for speed/resolution.
This capillary is also suitable for other applications where no EOF is desired. Thanks to the polyacrylamide coating, no EOF is present. The capillary can be used with both UV and LIF detection systems but capillary life is impacted when used with diodearray detection systems.

The SDS-Gel MW Analysis Kit is designed for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product.

This includes:
• Separation Capillary, 57 cm x 50 µm ID, bare fused-silica, (2)
• SDS Gel Separation Buffer, 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• SDS Protein Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• SDS-Gel MW Analysis Guide (1)

SDS-MW Gel Buffer is a 4-pack of the SDS-MW gel used in the SDS-MW Kit (Part Number 390953) and the IgG Purity and Heterogeneity Kit Assay (Part Number A10663).

SDS-MW gel designed for the separation and sizing of protein samples, utilizing SDS complexes as a replaceable gel matrix.

Loading Solution for DNA sequencing and fragment analysis samples

Contains cartridge body, 100 x 800 µm aperture, 100 x 200 µm aperture, tubing kit, nuts, ferrules, and O-rings. This cartridge body comes with 60 cm coolant tubing installed and also includes 30, 40, and 50 cm tubing.

Separation Buffer for performing capillary Isoelectric Focusing.
(100 mL).

Gel used in the cIEF application for experimentally determining a molecule's isoelectric point (pI) and the various charge variants of the molecule.

pI Marker Kit features synthetic peptides for high resolution and quantitative reproducibility in calculating protein pI. The combination of the advanced cIEF methods, the synthetic pI markers, and the PA 800 Plus system results in cIEF separations with the highest level of precision for pI determination and isoform quantitation of your sample.

This kit includes:
• Five vials containing 240 µL each of peptides with the following pI values: pI 4.1, pI 5.5, pI 7.0, pI 9.5, pI 10.0.
This volume enables 100 cIEF runs.

The eCAP dsDNA 1000 Kit is designed for DNA fragment pattern recognition, quantitation of PCR fragments, and molecular sizing on SCIEX CE Systems.
This chemistry utilizes linear polyacrylamide in solution to form a sieving gel. A neutral capillary provided with this kit has a stable, physically bonded polyacrylamide coating that eliminates electroosmotic flow (EOF) and suppresses interactions of the sample with the capillary wall. This chemistry utilizes a standard Tris-Borate EDTA buffer and allows the addition of intercalating dyes like ethidium bromide or our proprietary EnhanCE™ to improve resolution and increase sensitivity.
This kit is ideal for the analysis of double-stranded DNA fragments from 72 to 1000 base pairs as well as for the analysis of PCR products, which can be analyzed directly with no sample clean-up. Applications of this kit include gene expression analysis (competitive PCR), and genotyping experiments.
The system is compatible with both UV and LIF detection, but LIF is recommended for most applications. For LIF applications, we recommend the use of the intercalating dye EnhanCE. This kit is not compatible with PDA detection.

Kit provides reagent volumes for analysis of 100 samples and includes:
> 100 µm I.D. 65 cm, 2 units neutral capillaries (DNA),
> vials of replaceable polyacrylamide gel,
> test mix consisting of HaeIII restriction digest 174 DNA containing 11 fragments from 72 bp to 1,353 bp,
> and instructions and methods for system set-up and analysis.