PRESENS

SFR vario v2 Shake Flask Reader for pH, DO, OUR and biomass

Reference: PRE-200001689

The SFR vario offers online monitoring of oxygen, biomass and pH or CO2 - simultaneously. Online measured biomass data can be correlated with parameters like optical density, cell dry weight, or cell concentration.

This way it is possible to get real-time information on e. g. OD600 development. The device optics can read out one oxygen and one pH or CO2 (CO2 Technical Data) sensor spots - integrated in the ready-to-use cultivation vessels - and also comprise a dedicated optical set-up for biomass monitoring. The oxygen uptake rate (OUR) can be calculated from the slope of the online oxygen measurements. The system has two long-lasting, rechargeable batteries, and is compatible with any standard shaking incubators. Up to 4 SFR vario can be controlled from the SFR vario software. Measurement data are transferred wirelessly via Bluetooth to a PC / notebook.

- Simultaneous real-time measurement of O2, biomass & pH or CO2
- Automatic OUR calculation
- Online measurement of optical density, cell dry weight, cell concentration by correlation with biomass measurements
- Parallel measurements in up to 4 shake flasks
- Wireless data transfer enables easy integration
- Bioprocess development & media optimization

SFR vario v2 consists of :
- SFR vario v2
- 2 rechargeable batteries
- charge adapter cord with US / EU / UK compatible plug
- USB Bluetooth adapter for communication to PC
- screw driver and mounting screws
- Getting Started

This device comprises rechargeable batteries (accumulators) for self-contained
power supply. Rechargeable batteries may be replaced by the manufacturer only.
Rechargeable batteries are subject to normal wear which is more or less
pronounced depending on operating and storage conditions. This normal wear is
no defect which is covered by the device warranty.

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SFR vario v2 Shake Flask Reader for pH, DO, OUR and biomass

Technical information

Specifications	Oxygen	pH*	Biomass

Measuring range	0 – 100 % O2	5.5 – 8.0 pH	Optical Density OD600 1 - 80
Response time (t90) at 25 °C	< 60 sec.	< 60 sec.	-
Resolution	± 0.01 % O2 at 0.21 % O2 ± 0.1 % O2 at 20.9 % O2	± 0.01 pH at pH = 7**	Depending on culture
Accuracy	± 0.05 % O2 at 0.2 % O2 ± 0.4 % O2 at 20.9 % O2	± 0.1 pH at pH = 7 with one-point adjustment ± 0.2 pH at pH = 7 with pre-calibration	Depending on culture
Drift	< 0.01 % O2 per day (sampling interval of 1 min.)	< 0.01 pH per day (sampling interval of 1 min.)	Depending on culture
Properties
Temperature range	from + 5 to + 50 °C
Compatibility	Aqueous solutions, ethanol (max. 10 % v/v), methanol (max. 10 % v/v), pH 2 - 10
Cross-sensitivity	Typically no cross-sensitivity in culture media	Reduced to ionic strength (salinity); a high concentration of small fluorescent molecules in the visible range can interfere
Sensor flasks are delivered irradiated

* provided Sensor Flasks are used without further handling in physiological solutions
** at 100 rpm & in cell culture media