The SDS-Gel MW Analysis Kit is designed for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product.

This includes:
• Separation Capillary, 57 cm x 50 µm ID, bare fused-silica, (2)
• SDS Gel Separation Buffer, 140 mL
• SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL
• SDS Protein Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL
• Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL
• Acidic Wash Solution, 0.1 N HCl, 100 mL
• Basic Wash Solution, 0.1 N NaOH, 100 mL
• SDS-Gel MW Analysis Guide (1)

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