What is a PCR cabinet?
A PCR cabinet or PCR workstation is a vertical laminar flow cabinet that is adjusted for the use of PCR equipment. It provides a downflow of filtered air to protect your experiment from cross-contamination with air contaminants. PCR cabinets always have an UV lamp to decontaminate the cabinet from RNA and DNA between 2 PCR cycles.
What is the difference between a PCR cabinet and a vertical laminar flow cabinet?
A PCR cabinet is in principle the same as a vertical laminar flow cabinet, but with a few adjustments:
- Reduced height: when doing PCR, you don’t need a lot of height. In fact the manipulations around PCR involve mainly pipetting, which is done at work surface height.
- Optimized for quick UV decontamination. UV decontamination is very efficient to destroy RNA and DNA. For that reason, it is useful to have a quick method of activating the UV lamp between 2 PCR cycles. During the UV decontamination, the sash window must be closed to protect the operator. A PCR cabinet has a sash window with hinges that can easily be closed. When the sash window is closed, the UV lamp is automatically activated to start the UV decontamination.
- Electrical sockets and other items that are not resistant against long routine UV exposure are not present in PCR cabinets.
- PCR cabinets typically also have shelf close to the UV lamp. Pipets can be put on the shelf for decontamination.
What is the difference between a PCR cabinet and a Dead Air Box?
Both cabinet types have an UV lamp and are optimised for a quick activation of UV decontamination. A PCR cabinet or PCR workstation has a downflow of filtered air, that protects the experiment against cross contamination with the air. A dead air box does not have this. A dead airbox is simply a cabinet with a UV lamp, and does not offer any form of protection of your experiment against air contaminants when the cabinet is open.
PCR-4A1 Airstream® Polymerase Chain Reaction Cabinet, 1.2m
Item No. 144241
ESCO MICRO PTE 2150005 PCR cabinets
PCR-3A1 Airstream® Polymerase Chain Reaction Cabinet, 0.9m
Item No. 154100
ESCO MICRO PTE 2150001 PCR cabinets