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Step 4. Cell expansion

Multiplying the modified immune cells leads to billions of cells.
Maximize cell density and maintain quality is a constant concern.
An appropriate cell culture environment is critical to cell expansion efforts, and each stage of bioprocessing is influenced by the steps preceding it.
I.e. choosing the right medium and feed for your flasks or bioreactors to achieve maximum cell health, density and productivity.
Your challenges

  • Automate and scale up cell production?
  • Maintain Biological Integrity? Robust, safe and reliable solution for easy aseptic manipulations of mammalian cells (including during viral or pathogen steps)?
  • How easy and accurate is your cell culture media health & metabolic indicators monitoring?
  • Is your cell counting and viability analysis method automated, easy and reliable? Can you answer data integrity questions from auditors?
Contact our expert

Relevant Webinars

Goldilocks & the Three Cells: The Art of Monitoring & Managing Cell Cultures - Beckman Coulter
Too acidic? Overly alkaline? Or just right? Culturing cells is a subtle, dynamic process that requires constant monitoring and adjustment to maintain a “biological Goldilocks zone (BGZ).” This zone represents the delicate balance between cells, cell media, reagents and growth conditions.
In this webinar, Dr. Lenz explains how cell volume data can be used as a surrogate means of monitoring other aspects of cell viability related to the “BGZ.” In particular, he examines how monitoring cell volume can be helpful for assessing cell activation and proliferation – a critical factor in CAR-T cell research. After concluding his remarks, Dr. Lenz participates in a brief but informative Q&A with webinar attendees. (Total time: 37:35)

Evolution in the Manufacturing of Cellular Therapies - Beckman Coulter
Lessons learned and current solutions Immunotherapy is a powerful treatment that harnesses the body’s immune system in the fight against cancer. With optimized cell therapy, the result is an expanded population of T cells primed to recognize and eradicate malignant tumor cells that would otherwise escape immune detection. The webinar will discuss how cellular therapies have evolved, lessons learned through the evolution and current solutions available today. Learning Objectives: Commercialization of manufacturing- preparing a CAR Garage, lessons learned and how does it impact the patient. Automated functional testing- fully integrated unit operations taking the art out of science!
Monitoring important and critical parameters during cell expansion allows you to characterize your process and therefore simplify your scale-up afterwards.
The small-scale data set helps define the most optimal conditions to apply when increasing processing volumes.