Major Applications: For separating whole living cells.
An elutriation rotor is designed to separate and/or concentrate monodisperse suspensions of single cells or particles (approximately 2 to 50 µm in diameter) according to size. An elutriation rotor combines two separation technologies, and each cell in the chamber is acted upon by the two opposing forces:
- Centrifugal force: Driving it away from the axis of rotation. This is the process of sedimentation under the influence of a centrifugal force field.
- Fluid velocity: Driving it toward the axis of rotation—“counterflow elutriation." This is the process of separation by washing.
Separation takes place in a funnel-shaped elutriation chamber. While the rotor is spinning in the centrifuge, a suspension of cells is pumped at a preset flow rate from outside the centrifuge into the rotor to the narrow end of the elutriation chamber. As suspended cells are introduced into the chamber, they migrate according to their sedimentation rates to positions in the gradient where the effects of the two forces upon them are balanced.
Small cells with low sedimentation rates are quickly washed toward the axis of rotation. These cells are washed out of the chamber, up through the rotor, and out into a collection vessel. Somewhat larger or denser cells move through the chamber more slowly and reach equilibrium at the elutriation boundary. The largest or densest cells remain near the inlet to the chamber where centrifugal force and fluid velocity are high. By increasing the flow rate in gradual steps, successive fractions of increasingly large or dense cells can be washed out of the rotor and collected. Continued incremental increase of the flow rate will finally elutriate all cells from the chamber.
Includes: bypass chamber and supplies, and features a quick-release assembly which can be autoclaved for sterile operation. Order Elutriation Chamber(s) separately and choose Capacity:
Large (40 mL), Standard (4 mL), Sanderson (5.5 mL).
For use in: Avanti J-26 XP Series, Avanti J-26S Series and J6-MI centrifuges equipped with viewport door and strobe assembly, and discontinued J6-MC and Avanti J-20 XP Series already equipped with Elutriation Accessories.
To Upgrade an Existing Beckman Coulter Centrifuge:
For Beckman Coulter Avanti J-26 XP Series, order the appropriate kit listed below (order only one kit): Part Number 366562, 50/60 Hz.
- For use with Avanti J-26 XP. Kit includes Strobe Lamp/Control Assembly and Door Assembly with Viewport. Product 366563, 50/60 Hz.
- For use with Avanti J-26 XPI. Kit includes Strobe Lamp/Control Assembly and Door Assembly with Viewport.
Beckman Coulter Elutriator Rotors are for research and development use only. They have not been designed for use in diagnostic or therapeutic procedures.
Rotor Type Specialty
Maximum RPM 5.000 rpm
rMAX Large Chamber: 168 mm ; Standard Chamber: 125 mm
rMIN Large Chamber: 34.2 mm ; Standard Chamber: 86 mm
Materials Aluminum, Stainless steel