The eCAP dsDNA 1000 Kit is designed for DNA fragment pattern recognition, quantitation of PCR fragments, and molecular sizing on SCIEX CE Systems.
This chemistry utilizes linear polyacrylamide in solution to form a sieving gel. A neutral capillary provided with this kit has a stable, physically bonded polyacrylamide coating which eliminates electroosmotic flow (EOF) and suppresses interactions of the sample with the capillary wall. This chemistry utilizes a standard Tris-Borate EDTA buffer and allows the addition of intercalating dyes like ethidium bromide or our proprietary EnhanCE™ to improve resolution and increase sensitivity.
This kit is ideal for the analysis of double- stranded DNA fragments from 72 to 1000 base pairs as well as for the analysis of PCR products which can be analyzed directly with no sample clean-up. Applications of this kit include gene expression analysis (competitive PCR), and genotyping experiments.
The system is compatible with both UV and LIF detection, but LIF is recommended for most applications. For LIF applications, we recommend the use of the intercalating dye EnhanCE. This kit is not compatible with PDA detection.
> 100 µm I.D. neutral capillaries (DNA),
> vials of replaceable polyacrylamide gel,
> test mix consisting of HaeIII restriction digest 174 DNA containing 11 fragments from 72 bp to 1,353 bp,
> and instructions and methods for system set-up and analysis.