Analytical ultracentrifuges
Macromolecule characterization in solution
AUC is referred as the Orthogonal method used as reference for exosomes, AAV, viral vector, virus load characterization.
As an example, a current hot topic is the characterization of gene therapy formulations by AUC. In this Webinar, Amy Henrickson demonstrates analytical ultracentrifugation (AUC) methods, using multi-wavelength (MW) detection and heavy water density matching, to characterize Lipid Nanoparticle (LNP) and Adeno-Associated Virus (AAV) formulations.
Using an analytical ultracentrifuge to study sedimentation coefficients of viral vectors gives insight into vectors with an intact, therapeutic genome. Virus capsids packaged with a complete genome will travel through a gradient different than capsids with a partial genome or no genome. Understanding where fully packaged capsids travel (sedimentation coefficient) allows researchers to confidently make purifications with density-gradient ultracentrifugation during manufacturing. Looking forward, data gathered from an AUC during process development will influence manufacturing scalability.
Want to learn more about Analytical Ultracentrifugation? Take a more in-depth look at our AUC Resources Section.
- Enables characterization of samples in their native state
- A specialized ultracentrifuge with unique detection capabilities
- Monitors a wide variety of particles
- No standards required: relies on first principles of thermodynamics
AUC is referred as the Orthogonal method used as reference for exosomes, AAV, viral vector, virus load characterization.
As an example, a current hot topic is the characterization of gene therapy formulations by AUC. In this Webinar, Amy Henrickson demonstrates analytical ultracentrifugation (AUC) methods, using multi-wavelength (MW) detection and heavy water density matching, to characterize Lipid Nanoparticle (LNP) and Adeno-Associated Virus (AAV) formulations.
Using an analytical ultracentrifuge to study sedimentation coefficients of viral vectors gives insight into vectors with an intact, therapeutic genome. Virus capsids packaged with a complete genome will travel through a gradient different than capsids with a partial genome or no genome. Understanding where fully packaged capsids travel (sedimentation coefficient) allows researchers to confidently make purifications with density-gradient ultracentrifugation during manufacturing. Looking forward, data gathered from an AUC during process development will influence manufacturing scalability.
Want to learn more about Analytical Ultracentrifugation? Take a more in-depth look at our AUC Resources Section.
Optima AUC A/I, 8 Hole, Sales Group, Analytical Ultracentrifuge
Item No.
309355
BECKMAN COULTER INTL
C00710
Analytical ultracentrifuges