Bromelin is a proteolytic enzyme solution which can hydrolyse peptide bonds on proteins present on the red cell membrane.
Bromelin is a buffered extract prepared from the root of the pineapple plant which is suitable for use in serological methods. Bromelin is a proteolytic enzyme solution which can hydrolyse peptide bonds on proteins present on the red cell membrane. This results in a reduction of the surface charge of the membrane. This membrane modification is useful in serological testing since it leads to an enhancement of some antigen-antibody interactions and the elimination of others. In general, the reactions of Rhesus, ABO, Lewis and P system antibodies are enhanced by enzyme modification of red cells. By denaturing Fya , Fyb , M, N, S, s and Xga antigens, enzyme modification can diminish or eliminate the reactions of the corresponding antibodies. This reagent meets the requirements of the concerned standards and guidelines. Performance characteristics are mentioned in the release documents, which are supplied with the products upon request